Talk Title: Towards Standardization of Urine Proteomics and Peptidomics
Dr. Yamamoto is a Research Professor and Director of “All-in-One Urine Test” Project at the Niigata University. He is a pioneer of Kidney and Urine Proteomics with the background of nephrology and kidney pathology and molecular biology. He graduated at the School of Medicine, Niigata University (1974) and completed his PhD, Degree of Medical Sciences (D.M.Sc.) degrees (1981). He became the Professor of Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University (1999), the Director of Institute of Nephrology (2011) and the Director of Niigata University satellite Center of Innovation Program (2013). He organized the HUPO Kidney and Urine Initiative as the chair (2005). He was elected to a HUPO council member (2011) and the President of Japan HUPO (Japanese Proteomics Society, JProS) (2012).
Urinary proteins and proteins are derived from blood and urinary tract tissues, informing pathophysiological conditions of the body as biomarkers. HUPO kidney and Urine Initiative (Human Kidney and Urine Proteome Project, HKUPP) was established to apply proteomics to nephrology and a guide for urine collection and storage was proposed as a standard protocol (http://www.hkupp.org/). A protocol for protein and peptide preparation from frozen urine samples for proteomics and peptidomics, a comparative study of non-label quantitative urine proteome analyse, and a workflow for identification of peptides are proposed in this presentation. Progresses of biomarker discovery for kidney diseases and “Liquid biopsy” by combining several biomarkers for kidney nephron parts are introduced.
Urine proteomics and peptidomics may provide urine biomarkers not only for kidney and other urinary tract diseases, but also for systemic diseases, which may make it possible to discover all diseases by urine examination, “All-in-One Urine Test” in the near future.