Michael B. Yaffe, M.D., Ph.D.
Talk title: The Proteomics of Protein Kinase Signaling
Michael B. Yaffe, M.D., Ph.D., is the David H. Koch Professor of Science at MIT, where he is a Professor of Biology and Biological Engineering at the Koch Institute for Integrative Cancer Research. He is also a Senior Associate Member of the Broad Institute, and an Attending Surgeon/Surgical Intensivist in the Division of Acute Care Surgery, Trauma and Critical Care, Department of Surgery at Beth Israel Deaconess Medical Center, Harvard Medical School.
Dr. Yaffe received his B.S. in Materials Science and Engineering from Cornell University, and his M.D. and Ph.D. degrees from Case Western Reserve University, followed by advanced post-doctoral training with Prof. Lew Cantley at Harvard Medical School. Dr. Yaffe’s research focuses on the biology of the complex signaling pathways that cells use to respond to injury, inflammation, and DNA damage, particularly the role of protein kinases and modular binding domains in trauma, tumor development, and anti-cancer therapeutics. His laboratory uses a multi-disciplinary approach encompassing systems biology, proteomics, biochemistry, cell and structural biology and computation/bioinformatics. Dr. Yaffe is the Scientific Editor-in-Chief of Science Signaling and a member of the Editorial Boards of Molecular and Cellular Proteomics, and Cell Cycle.
Bert van de Kooij, Konstantin Krismer, Thomas Bernwinkler, Pau Creixell, Mun Kyung Hwang, Simon Gordonov, Anne Van Vlimmeren, Amanda Del Rosario, Tobias Ehrenberger, Ian G. Cannell, Frank B. Gertler, Douglas A. Lauffenburger, and Michael B. Yaffe
David H. Koch Institute for Integrative Cancer Research, Departments of Biological Engineering and Biology, Massachusetts Institute of Technology, Cambridge, MA USA
In this talk I will summarize our progress elucidating the phosphorylation motifs for the entire human kinome using oriented peptide library screening approaches and computational analysis of mass spectrometry datasets. I will showcase the current version of Scansite (https://scansite4.mit.edu/4.0/#home), a free web-based bioinformatics tool that links protein phosphorylation sites to their likely upstream kinases, allowing construction of signaling pathways in silico. Finally, I will present new data highlighting motifs and substrates of poorly understood families of mitotic kinases, and show unexpected roles for the DNA damage-responsive kinases Chk1, Chk2, and MK2 in a cytoskeletal-mediated adaptive response to genotoxic stress.