Talk title: Proteomics, Embracing The Chaos
Professor at the Wellcome Trust Institute for Cell Biology (University of Edinburgh) and the Institute for Biotechnology (TU Berlin), Juri Rappsilber combines chemistry, biology & informatics to develop qualitative & quantitative mass spectrometry methods. He uses these methods to study protein structure and protein–protein interactions in complex mixtures. This work started as a PhD student with Matthias Mann at EMBL Heidelberg and continued throughout his postdoc with Matthias in Odense. In 2003, Juri started as an independent PI at IFOM Milan. Since 2006, Juri is in Edinburgh and since 2011 also in Berlin. Juri is particularly indebted to the Wellcome Trust for funding his work.
As a PhD student I learned the three most important indications for the function of an uncharacterised protein: location, location, location. Or is it? As analysis depth increases so does the number of out-of-context proteins identified at any location in the cell investigated. Of course, analysis by proteomics requires prior isolation of a cellular structure/organelle. Are cells tidy and experiments dirty? Here we analyse chromatin by proteomics and challenge the black/white logic of proteomic lists and GO annotations. Interestingly, this departure shows a more direct root to protein function and may affect our view of the cellular interior.