Talk Title: Bioprocess Monitoring using Quantitative Proteomics, Glycomics and Glycoproteomics.
Jonathan received his PhD in Analytical Chemistry from Dublin City University in 2007. Jonathan then moved to NIBRT – The National Institute for Bioprocessing Research and Training, working under the mentorship of Prof. Pauline M. Rudd within her GlycoScience Laboratory. In 2010, Jonathan was appointed the John Hatsopoulos Research Scholar within the Barnett Institute of Chemical and Biological Analysis at Northeastern University, Boston, working under the mentorship of Prof. Barry L. Karger. Jonathan returned to NIBRT in 2012 and is the Principal Investigator of the NIBRT Characterization and Comparability Laboratory and an Associate Professor in the School of Chemical and Bioprocess Engineering at University College Dublin.
Bioprocess Monitoring using Quantitative Proteomics, Glycomics and Glycoproteomics.
As routinely used in the field of basic research, quantitative LC-MS based proteomics offers immense potential to better understand biopharmaceutical production using industrial scale mammalian cell culture. A number of examples of the application of quantitative proteomics for bioprocess monitoring will be described. Label free quantitative proteomics was applied to identify markers of cell physiology over the duration of a perfusion bioprocess. Having first identified candidates to monitor apoptosis and necrosis in CHO cells using small scale models, translation into a targeted platform with subsequent verification and validation on the industrial scale were then performed demonstrating superior performance as compared to currently employed assays. Isobaric labelling using tandem mass tags to identify potential markers in response to systematic alterations in bioprocess conditions and associated implications for product quality will also be presented. Finally, the investigations into the CHO cell glycome and its response following exposure to leachable compounds from single use bioreactors will be described.