Talk Title: Subcellular proteomics and friends; finding lipid raft-dependent RNA-binding proteins that regulate exosome microRNA cargo selection
Associate Professor Michelle Hill obtained her PhD at the University of Queensland (UQ) on insulin signalling using phosphoproteomics. Her postdoctoral work in Switzerland, Ireland, and at Institute for Molecular Bioscience UQ focussed on various aspects of signalling using subcellular proteomics. With a passion for translational proteomics research, she established the Cancer Proteomics Group at the UQ Diamantina Institute in 2009. Through interdisciplinary collaborations, the group has successfully developed a laboratory test for amyloidosis subtyping, and is in the process of developing a blood test for oesophageal adenocarcinoma detection. Michelle is an elected member of the international Human Proteome Organisation (HUPO) Council, and inaugural chair of the Queensland Mass Spectrometry Symposium. In April 2017, Michelle’s research group will begin a new chapter at the QIMR Berghofer Medical Research Institute, where she intends to expand her fledgling lipidomics and chemical analysis capabilities.
Subcellular proteomics and friends; finding lipid raft-dependent RNA-binding proteins that regulate exosome microRNA cargo selection
Michelle M Hill
1. The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia
2. QIMR Berghofer Medical Research Institute, Brisbane, Australia
Extracellular vesicles (EV) including exosomes and microvesicles mediate intercellular communication by delivering functional biomolecules including microRNA (miRNA) to recipient cells. We previously linked the putative tumour suppressor action of cavin-1/PTRF in prostate cancer with altered EV miRNA and lipid raft membrane micodomain proteome. Cavin-1 selectively reduced the EV levels of a subset of miRNAs, independent of their cellular levels. We hypothesized that selective miRNA loading is mediated by cavin-1 induced lipid raft RNA-binding proteome changes. To test this hypothesis and identify the specific RNA-binding protein, we applied subcellular proteomics, EV small RNA sequencing, computational and multiple cell biology techniques to identify a cavin-1 regulated RNA binding protein and a novel RNA motif that could mediate the cargo loading. Overall, this presentation will demonstrate an integrative subcellular systems biology approach to address key molecular mechanisms