Instrumentation and Methods for the identification and Sequence aNALYSIS OF (a) intact proteins on a ChromatograpHic time-scale and (2) Characterization tumor specific phosphoPeptides for immunotherapy of cancer
Donald F. Hunt is a University Professor of Chemistry and Pathology at the University of Virginia and pioneered efforts to develop methods and mass spectrometry instrumentation that set the standard for ultrasensitive detection and characterization of proteins and peptides. These contributions continue to underpin the whole field of proteomics and have had a dramatic impact on research in immunology, cell signaling, cell migration, chromatin biology and immunotherapy of cancer. He is a Fellow of the American Academy of Arts and Sciences and won the American Chemical Society Award in Analytical Chemistry this past April. He is a consultant to both Thermo-Fisher Inc. and Agenus Inc. and has now published more than 400 papers with 44,716 citations. He has a Google Scholar h-index of 110 and an i10-index of 364.
Departments of Chemistry and Pathology, University of Virginia, Charlottesville, VA 22904
This lecture will focus on data generated with an ion source that facilitates simultaneous generation of positively charged sample ions by electrospray ionization and negatively charged reagent ions for both electron transfer dissociation (ETD) and ion-ion proton transfer (IIPT) reactions on Orbitrap mass spectrometers. Implementation of multiple C-trap fills for enhanced sensitivity will be discussed and both parallel peak parking, and ion ejection strategies to facilitate protein separation and enhanced sequence coverage of intact proteins will be described. Use of IIPT/ETD facilitates near complete sequence coverage on many intact proteins, including antibodies, and is ideally suited for locating multiple posttranslational modifications on the same protein molecule. Also discussed is the identification of posttranslationally modified, Class I MHC peptides that trigger central memory T-cells to kill cancer cells.