Proteomic exploration of the scope, dynamics, and stoichiometry of lysine acetylation
Lysine acetylation is a key regulatory posttranslational modification. Function of lysine acetylation is most extensively studied in the context of epigenetic regulation of gene transcription via acetylation of histones. Recent mass spectrometry (MS)-based proteomic studies have greatly expanded our knowledge of this modification. Our laboratory is using MS-based quantitative proteomics to map the scope of acetylation in diverse organisms and to investigate its dynamic regulation in response to genetic and environmental perturbations. Furthermore, we developed novel proteomic methods to accurately quantify stoichiometry of acetylation on a proteome-wide scale. We showed that acetylation can occur through both enzymatic and non-enzymatic mechanisms, and our results suggest an important function of sub-cellular compartmentalization in the evolution of acetylation signaling in eukaryotes. Our systems-wide investigations provide important insights in to the mechanisms, dynamics, evolution, and specificity in acetylation signaling. I will discuss our recently published and ongoing efforts in understanding acetylation signaling.
Dr. Chuna Choudhary is a professor and group leader at the Novo Nordisk Foundation Center for Protein Research at the University of Copenhagen in Denmark. He carried out his doctoral studies at the University of Munster in Germany and his postdoctoral training at the same university as well as at the Max Planck Institute for Biochemistry in Munich, Germany. His laboratory is interested in studying posttranslational modification-based cell signaling networks using quantitative mass spectrometry-based approaches. His group combines quantitative proteomics with cell and molecular biology approaches to investigate the dynamics and functions of lysine acetylation and ubiquitylation in cellular signaling networks. Dr. Choudhary has widely published in high-ranked journals and received several honors and awards, including the EMBO Young Investigator award, the Danish Cancer Society Junior Researcher award, and the ERC Consolidator grant.