Full Professor of Biochemistry,
Head of Clinical Chemistry Facility
Talk Title: Perspectives of proteomics in laboratory medicine and total lab automation
His international publication record is represented by over 180 contributions on journal with peer review system for a total impact factor above 570, his h-index is 42 based on 5174 citations (Google Scholar, Apr. 2017).
He has acted on the behalf of IFCC and SIBioC (Italian Society of Clinical Chemistry and Clinical Molecular Biology) as expert for the development of the project C50-P “Mass Spectrometry in the Clinical Laboratory: General Principles and Guidance; Proposed Guideline”, approved document by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS) and IFCC.
Selected pubblications 2017-2012
1: Davoli A, Greco V, Spalloni A, Guatteo E, Neri C, Rizzo GR, Cordella A, Romigi A, Cortese C, Bernardini S, Sarchielli P, Cardaioli G, Calabresi P, Mercuri NB, Urbani A, Longone P. Evidence of hydrogen sulfide involvement in amyotrophic
lateral sclerosis. Ann Neurol. 2015 Apr;77(4):697-709. doi: 10.1002/ana.24372. Epub 2015 Mar 13. PubMed PMID: 25627240.
2: Greco V, Pieragostino D, Piras C, Aebersold R, Wiltfang J, Caltagirone C, Bernardini S, Urbani A. Direct analytical sample quality assessment for biomarker investigation: qualifying cerebrospinal fluid samples. Proteomics. 2014 Sep;14(17-18):1954-62. doi: 10.1002/pmic.201300565. Epub 2014 Aug 8. PubMed PMID: 25044759.
3: Alberio T, Bondi H, Colombo F, Alloggio I, Pieroni L, Urbani A, Fasano M. Mitochondrial proteomics investigation of a cellular model of impaired dopamine homeostasis, an early step in Parkinson’s disease pathogenesis. Mol Biosyst. 2014
Jun;10(6):1332-44. doi: 10.1039/c3mb70611g. Epub 2014 Mar 28. PubMed PMID: 24675778.
4: Colanzi A, Grimaldi G, Catara G, Valente C, Cericola C, Liberali P, Ronci M, Lalioti VS, Bruno A, Beccari AR, Urbani A, De Flora A, Nardini M, Bolognesi M, Luini A, Corda D. Molecular mechanism and functional role of brefeldin A-mediated
ADP-ribosylation of CtBP1/BARS. Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9794-9. doi: 10.1073/pnas.1222413110. Epub 2013 May 28. PubMed PMID: 23716697; PubMed Central PMCID: PMC3683763.
5: Bortolato M, Godar SC, Melis M, Soggiu A, Roncada P, Casu A, Flore G, Chen K, Frau R, Urbani A, Castelli MP, Devoto P, Shih JC. NMDARs mediate the role of monoamine oxidase A in pathological aggression. J Neurosci. 2012 Jun
20;32(25):8574-82. doi: 10.1523/JNEUROSCI.0225-12.2012. PubMed PMID: 22723698; PubMed Central PMCID: PMC3417343.
Perspectives of proteomics in laboratory medicine and total lab automation.
1Institute of Biochemistry and Clinical Biochemistry, Catholic University of the “Sacred Heart”, Rome; 2Clinica Chemistry Department, Fondazione Policlinico Gemelli, Rome; 3IRCCS-Fondazione S. Lucia, Rome; ITALY
Laboratory medicine is swiftly moving forward in the field of total lab automation associating hub centers, main operations able of running millions of test per years, with spoke structures distributed on the territory. In the last five years a number of proteomics initiatives have been opening new routes in the development of new clinical deliverables. These associate the key concepts of Proteomics which links together two fundamental ideas: the thorough investigation of protein structures with a multifactorial integration of data. Nevertheless the sustainability of these diagnostic products in the current healthcare systems and the current driving force toward more efficient clinical laboratory settings are often not fully discussed. This presentation will touch and possibly integrate these two worlds providing some examples from one of the largest clinical chemistry operation facility in Italy, the Gemelli Hospital clinical laboratory, running about 6.5 Millions tests per year. The potentiality of some Proteomics deliverables will be introduced and discussed in the light of the current analytical performances of different technological platforms and cost effectiveness.